Facilities

Biological Safety Cabinet class II A2 : Thermofisher Scientific

BSC class II A2 is certified to work on cancer cells, viral strain, and avirulent bacterial strain by Guwahati Biotech Park and Institutional Committee for Biosafety, KaviKrishna Laboratory

BSC class 3 cabinet : Clean air

This is used to grow and culture anaerobic micro-organisms. We also use this jar to study the cellular behavior and host pathogen interaction of cancer stem cell and Mycobacterum tuberculosis under hypoxic condition.

Soxlet Apparatus

Extraction of different parts such as leaves, root and seed of plants. We use this to find out squalene related isoprenoid molecules from the plant extract which can be use to target cancer stem cell

Western blotting equipments

Western blotting is a widely used technique by research and clinical labs to detect proteins with their specific antibody. It is used as a general method to identify the presence of a specific single protein within a complex mixture of proteins. A semi-quantitative estimation of a protein can be derived from the size and color intensity of a protein band on the blot membrane.

Elisa Plate Reader

The ELISA reader is an instrument which is used to detect the presence of protein in a liquid sample.ELISA Plate reader is used to detect and process biological and chemical data using absorbance, luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. These plate readers are used in drug discovery, research and bioassay validation and biopharmaceutical manufacturing.

We are using this plate reader mainly to observe the expression of different stemness proteins in cancer cells or mesenchymal stem cells

-80° Freezer

This freezer is used for long term storage of bacterial isolation, proteins and RNAs. They come with precise temperature monitoring making them ideal for storing vaccines, blood, plasma, reagents and other temperature-sensitive items.

Cold Centrifuge

We are using the cold centrifuge specifically for animal cell culture. It is used to spin 5ml upto 50 ml sample at minimum 4degree centigrade

Micro Centrifuge

A microcentrifuge, also called a microfuge, is used to spin small (2 ml or less) liquid samples at high speeds (generally tens of thousands times g-force). It is used for Cell culture and western blotting purpose

It is specifically used for molecular biology assays. E.g. during DNA/RNA isolation

HPLC

HPLC or High-performance liquid chromatography has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion.

Can be availed from Guwahati Biotech Park

FACs Sorter

This is a very important and costly machine for cell culture work. To sort a specific population such as cancer stem cell, side population and to analyse the cells this machine is used.

Can be availed from Guwahati Biotech Park

In vivo animal house

We have black six and white balb-C mice in our in vivo facility, which is situated at Guwahati University. We regularly maintain this facility for our in vivo serial transplanation assays.

It is situated at Guwahati University

Confocal Microscope

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.

Anaerobic Jar

This is used to grow and culture anaerobic micro-organisms. We also use this jar to study the cellular behavior and host pathogen interaction of cancer stem cell and Mycobacterum tuberculosis under hypoxic condition.

CO2 Incubator

This is a must needed facility to maintain cell and tissue culture. It creates a natural environment for the cells to grow properly. It maintains 37degree centigrade with 5% Co2

Electrophoresis Gel Tank

This is to perform sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). It is a technique widely used in molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. This is a part of our western blotting experiment to study the expression of stemness protein in cancer cells or mesenchymal stem cells

Magnetic Cell sorter

This is a very important instrument, use to sort a specific cell population from a general cell population using marker. We use this to sort specific population of cancer stem cell and mesenchymal stem cell for our cancer and TB study

Analytic Balance

Analytical weight balance in an instrument use to measure the weight of a substance. Analytical balance is more accurate with a sensitivity of 0.1 g or lower. The balance is enclosed in a glass case to avoid air draft

pH meter

pH meter is an instrument used to measure pH of a given solution and prepare buffer for biological research. pH meter measures the hydrogen ion concentration using an ion sensitive electrode.